Gilson Connect
Processing thousands of wildlife samples for next-generation sequencing can overwhelm manual workflows. This case study shows how the Peery Wildlife Ecology and Conservation Lab automated key NGS steps with PIPETMAX® 268, reducing errors, doubling throughput, and enabling their team to scale conservation genomics research more efficiently.
This application note outlines the automation of the Agencourt® AMPure® XP PCR purification system using Gilson’s PIPETMAX® 268. Designed to streamline PCR cleanup for next-generation sequencing workflows, the protocol uses SPRI magnetic beads to remove contaminants and isolate DNA fragments over 100 bp. The automated method improves reproducibility and throughput, processing up to 96 samples per run with a customizable magnetic bead separator. Applied to E. coli K12 gDNA libraries, the system produced high-quality fragments between 700–2000 bp with no contamination. The protocol is flexible, allowing users to adjust variables like reagent volumes, incubation times, and wash steps to fit diverse lab needs.
Reliable and reproducible library preparation is critical for successful next generation sequencing (NGS). In this poster, We compare sequencing results for E. coli whole genome sequencing libraries prepared with the Illumina Nextera XT kit, with liquid handling carried out either manually or automated with PIPETMAX 268.
Next generation sequencing (NGS) became essential in routine HLA typing due to its efficiency in the context of resolution, costs, and throughput. To understand the required pipetting steps during NGS library preparation, we integrated the manual 96-channel pipetting system PLATEMASTER into our NGS workflow.
This application note describes the setup and execution of automation of the Illumina® Nextera® XT DNA Library Preparation Kit using the PIPETMAX 268 automated liquid handler. Five different scripts have been created and are described in detail in this document.
Physicians need test kits to identify all of the genetic mutations of a condition at once so that they can initiate the right treatment. In this app note, we describe how the PIPETMAX 268 automated liquid handler can be used within a BRCA MASTR Dx molecular diagnostic assay.
In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAMlabeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX 268. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.
